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Cytotoxicity of amyloid fibrils derived from hen lysozyme with different properties on SH-SY5Y and cytoprotective effect of prunus mume extracts
https://kawasakigakuen.repo.nii.ac.jp/records/305
https://kawasakigakuen.repo.nii.ac.jp/records/305048cae5e-9315-4c72-b15b-d811915686f7
名前 / ファイル | ライセンス | アクション |
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C 1-020-026 (1.1 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-08-10 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Cytotoxicity of amyloid fibrils derived from hen lysozyme with different properties on SH-SY5Y and cytoprotective effect of prunus mume extracts | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hen lysozyme | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Protein misfolding | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Dementia | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hereditary lysozyme amyloidosis | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_1843 | |||||
資源タイプ | other | |||||
内容種別 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Original Article | |||||
著者 |
Ryohei KONO
× Ryohei KONO× Sachiko NOMURA× Yoshiharu OKUNO× Tomoko UTSUNOMIYA× Hirotoshi UTSUNOMIYA |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Protein misfolding is associated with formation of amyloid fibrils. Hen egg lysozyme, well-known for its structural properties, has been shown by atomic force microscopy to form needle-shaped amyloid fibrils under acidic conditions with high temperature. When disulfide bonds of hen lysozyme were reduced by reductant TCEP, it formed noodle-shaped amyloid fibrils. Difference in thioflavin T and 8-Anilino-1-naphthalenesulfonic acid (ANS) fluorescence of these fibrils suggested difference in structural properties. The needle-shaped amyloid fibrils exerted cytotoxicity on human neuroblastoma SH-SY5Y cells, but noodle-shaped amyloid fibrils did not. The reason for cytotoxicity being exerted only by needle-shaped amyloid fibrils was thought to be due to the difference in properties, such as fibril surface and inter-/intra-molecule structure. This finding indicates the importance of studying the interaction between the amyloid fibril surface and inter-/intra-molecular structural properties and cells. We also investigated whether Prunus mume extracts protect SH-SY5Y cells from hen lysozyme amyloid-induced toxicity. The Prunus mume seed extract protected the cells from needle-shaped amyloid toxicity. Prunus mume could therefore potentially prevent amyloid-related disease. | |||||
書誌情報 |
en : COGNITION & REHABILITATION 巻 1, 号 1, p. 20-26, 発行日 2020-12 |
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出版者 | ||||||
言語 | ja | |||||
出版者 | 大阪河﨑リハビリテーション大学 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 2436-1097 |