{"created":"2023-05-15T08:48:51.423785+00:00","id":305,"links":{},"metadata":{"_buckets":{"deposit":"fdbfd05a-86a0-4c38-a3c6-1830f038c202"},"_deposit":{"created_by":4,"id":"305","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"305"},"status":"published"},"_oai":{"id":"oai:kawasakigakuen.repo.nii.ac.jp:00000305","sets":["33:34"]},"author_link":["377","374","378","379","380"],"control_number":"305","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"26","bibliographicPageStart":"20","bibliographicVolumeNumber":"1","bibliographic_titles":[{"bibliographic_title":"COGNITION & REHABILITATION","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Protein misfolding is associated with formation of amyloid fibrils. Hen egg lysozyme, well-known for its structural properties, has been shown by atomic force microscopy to form needle-shaped amyloid fibrils under acidic conditions with high temperature. When disulfide bonds of hen lysozyme were reduced by reductant TCEP, it formed noodle-shaped amyloid fibrils. Difference in thioflavin T and 8-Anilino-1-naphthalenesulfonic acid (ANS) fluorescence of these fibrils suggested difference in structural properties. The needle-shaped amyloid fibrils exerted cytotoxicity on human neuroblastoma SH-SY5Y cells, but noodle-shaped amyloid fibrils did not. The reason for cytotoxicity being exerted only by needle-shaped amyloid fibrils was thought to be due to the difference in properties, such as fibril surface and inter-/intra-molecule structure. This finding indicates the importance of studying the interaction between the amyloid fibril surface and inter-/intra-molecular structural properties and cells. We also investigated whether Prunus mume extracts protect SH-SY5Y cells from hen lysozyme amyloid-induced toxicity. The Prunus mume seed extract protected the cells from needle-shaped amyloid toxicity. Prunus mume could therefore potentially prevent amyloid-related disease.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_description_6":{"attribute_name":"内容種別","attribute_value_mlt":[{"subitem_description":"Original Article","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"大阪河﨑リハビリテーション大学","subitem_publisher_language":"ja"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2436-1097","subitem_source_identifier_type":"PISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ryohei KONO","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sachiko NOMURA","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshiharu OKUNO","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tomoko UTSUNOMIYA","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hirotoshi UTSUNOMIYA","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-08-10"}],"displaytype":"detail","filename":"C 1-020-026.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"C 1-020-026","url":"https://kawasakigakuen.repo.nii.ac.jp/record/305/files/C 1-020-026.pdf"},"version_id":"b7011737-513d-43c4-8318-c94f2b29fb5d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Hen lysozyme","subitem_subject_scheme":"Other"},{"subitem_subject":"Protein misfolding","subitem_subject_scheme":"Other"},{"subitem_subject":"Dementia","subitem_subject_scheme":"Other"},{"subitem_subject":"Hereditary lysozyme amyloidosis","subitem_subject_scheme":"Other"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"other","resourceuri":"http://purl.org/coar/resource_type/c_1843"}]},"item_title":"Cytotoxicity of amyloid fibrils derived from hen lysozyme with different properties on SH-SY5Y and cytoprotective effect of prunus mume extracts","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cytotoxicity of amyloid fibrils derived from hen lysozyme with different properties on SH-SY5Y and cytoprotective effect of prunus mume extracts","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"4","path":["34"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-08-10"},"publish_date":"2021-08-10","publish_status":"0","recid":"305","relation_version_is_last":true,"title":["Cytotoxicity of amyloid fibrils derived from hen lysozyme with different properties on SH-SY5Y and cytoprotective effect of prunus mume extracts"],"weko_creator_id":"4","weko_shared_id":-1},"updated":"2023-10-02T00:52:30.982748+00:00"}