| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-12-18 |
| タイトル |
|
|
タイトル |
Recombinant human neuroblastoma sh-sy5y cells overexpressing α-synuclein form amyloid aggregates in seed-dependent and seed-independent manners |
|
言語 |
en |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
α-synuclein |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
SH-SY5Y |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Parkinson’s disease |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Dementia with levy body |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Amyloid |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
article |
| ID登録 |
|
|
ID登録 |
10.69202/0002000171 |
|
ID登録タイプ |
JaLC |
| 内容種別 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
ORIGINAL ARTICLE |
| 著者 |
Ryohei KONO
Haruki FUJIHIRA
Yoshiharu OKUNO
Hirotoshi UTSUNOMIYA
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Alpha-synuclein (α-Syn) has prion-like properties and is one of the causative proteins of Parkinson’s disease (PD) and dementia with Lewy bodies (DLB). Neuronal loss is thought to result from amyloid aggregation caused by misfolding of protein and propagation of misfolded protein aggregate as seeds through the neural network. This may contribute to the progression of PD and DLB. Prevention of accumulation and propagation of amyloid and neuronal loss is therefore important in the prevention and slowing of the progression of PD and DLB. In this study, we aimed to establish a neuronal cell line stably overexpressing α-Syn and to clarify its ellular characteristics for the study of amyloid aggregation and aggregation inhibition. Through gene transfer and selection with the antibiotic G418, we obtained subclones derived from SH-SY5Y, the human euroblastoma cell line that overexpress α-Syn. The subclones were found to form amyloid aggregates in a markedly short period of time upon addition of α-Syn amyloid fibril-derived seeds. They also expressed βIII tubulin, a mature neuron marker, in long-term culture with differentiation induction by retinoic acid or brain-derived neurotrophic factor stimulation. The subclones are thus suggested to retain the ability to differentiate into mature neurons as well as the wild type. Furthermore, the subclones in long-term culture showed seed-independent aggregate formation, nuclear condensation, and fragmentation. This suggests that the subclones spontaneously form ggregates and undergo cell death under long-term culture due to high α-Syn expression levels. The α-Syn-overexpressing SH-SY5Y cell line established in this study is thought to be useful for research on α-Syn amyloid aggregate formation, inhibition of aggregate formation, propagation, and aggregate-associated cell death. Moreover, the study suggests the potential for use in research on neuronal dysfunction by inducing differentiation into mature neurons, and is therefore expected to be useful in preventing and slowing the progression of PD and DLB. |
|
言語 |
en |
| 書誌情報 |
en : COGNITION & REHABILITATION
巻 5,
号 1,
p. 9-17,
ページ数 9,
発行日 2024-12-20
|
| 出版者 |
|
|
出版者 |
Osaka Kawasaki Rehabilitation University |
|
言語 |
en |
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
2436-1097 |
p09-17_Ryohei KONO.pdf (3 MB)
