| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2021-08-10 |
| タイトル |
|
|
タイトル |
Cytotoxicity of amyloid fibrils derived from hen lysozyme with different properties on SH-SY5Y and cytoprotective effect of prunus mume extracts |
|
言語 |
en |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Hen lysozyme |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Protein misfolding |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Dementia |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Hereditary lysozyme amyloidosis |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_1843 |
|
資源タイプ |
other |
| ID登録 |
|
|
ID登録 |
10.69202/0000000305 |
|
ID登録タイプ |
JaLC |
| 内容種別 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Original Article |
| 著者 |
Ryohei KONO
Sachiko NOMURA
Yoshiharu OKUNO
Tomoko UTSUNOMIYA
Hirotoshi UTSUNOMIYA
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Protein misfolding is associated with formation of amyloid fibrils. Hen egg lysozyme, well-known for its structural properties, has been shown by atomic force microscopy to form needle-shaped amyloid fibrils under acidic conditions with high temperature. When disulfide bonds of hen lysozyme were reduced by reductant TCEP, it formed noodle-shaped amyloid fibrils. Difference in thioflavin T and 8-Anilino-1-naphthalenesulfonic acid (ANS) fluorescence of these fibrils suggested difference in structural properties. The needle-shaped amyloid fibrils exerted cytotoxicity on human neuroblastoma SH-SY5Y cells, but noodle-shaped amyloid fibrils did not. The reason for cytotoxicity being exerted only by needle-shaped amyloid fibrils was thought to be due to the difference in properties, such as fibril surface and inter-/intra-molecule structure. This finding indicates the importance of studying the interaction between the amyloid fibril surface and inter-/intra-molecular structural properties and cells. We also investigated whether Prunus mume extracts protect SH-SY5Y cells from hen lysozyme amyloid-induced toxicity. The Prunus mume seed extract protected the cells from needle-shaped amyloid toxicity. Prunus mume could therefore potentially prevent amyloid-related disease. |
|
言語 |
en |
| 書誌情報 |
en : COGNITION & REHABILITATION
巻 1,
号 1,
p. 20-26,
発行日 2020-12
|
| 出版者 |
|
|
出版者 |
大阪河﨑リハビリテーション大学 |
|
言語 |
ja |
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
2436-1097 |
C 1-020-026 (1.1 MB)
